Polymerase Chain Reaction

A step-by-step protocol for formaldehyde-assisted isolation of regulatory elements from Arabidopsis thaliana.

The control of gene expression by transcriptional regulators and other types of functionally relevant DNA transactions such as chromatin remodeling and replication underlie a vast spectrum of biological processes in all organisms. DNA transactions …

QuantPrime--a flexible tool for reliable high-throughput primer design for quantitative PCR.

BACKGROUND: Medium- to large-scale expression profiling using quantitative polymerase chain reaction (qPCR) assays are becoming increasingly important in genomics research. A major bottleneck in experiment preparation is the design of specific primer …