Abstract
Multiple transitions from insect to wind pollination are associated with polyploidy and unisexual flowers in (Ranunculaceae), yet the underlying genetics remains unknown. We generated a draft genome of , a representative of a clade with ancestral floral traits (diploid, hermaphrodite, and insect pollinated) and a model for functional studies. Floral transcriptomes of and of wind-pollinated, andromonoecious are presented as a resource to facilitate candidate gene discovery in flowers with different sexual and pollination systems. A draft genome of and two floral transcriptomes of and were obtained from HiSeq 2000 Illumina sequencing and de novo assembly. The de novo draft genome assembly consisted of 44,860 contigs (N50 = 12,761 bp, 243 Mbp total length) and contained 84.5% conserved embryophyte single-copy genes. Floral transcriptomes contained representatives of most eukaryotic core genes, and most of their genes formed orthogroups. To validate the utility of these resources, potential candidate genes were identified for the different floral morphologies using stepwise data set comparisons. Single-copy gene analysis and simple sequence repeat markers were also generated as a resource for population-level and phylogenetic studies.
Diego Mauricio Riaño-Pachón
Assistant Professor (MS3.2) in Computational, Evolutionary and Sistems Biology
I am a computational biologist/bioinformatician at the University of São Paulo, Campus Luiz de Queiroz (Piracicaba/SP, Brazil).